Pinealon
★ 55Pinealon (Glu-Asp-Arg tripeptide)
Russian neuroprotective sleep tripeptide
SleepAbout
Pinealon is a synthetic Glu-Asp-Arg (EDR) tripeptide from the Khavinson bioregulator family, developed at the St. Petersburg Institute of Bioregulation and Gerontology. It is proposed to enter cells and cross the nuclear membrane to bind gene promoter regions, influencing antioxidant and neurotrophic gene expression. The evidence base is dominated by Russian-language preclinical work and small clinical cohorts.
Mechanism
Proposed epigenetic regulation via direct binding to DNA promoter regions, modulating ERK 1/2 signaling and upregulating antioxidant and anti-apoptotic gene expression in neurons.
Dosage
beginner
- Amount
- 1.0 mg
- Frequency
- Once daily
- Route
- SubQ
- Duration
- 5 days
standard
- Amount
- 1.5 mg
- Frequency
- Once daily
- Route
- SubQ
- Duration
- 9 days (days 6-14)
advanced
- Amount
- 2.0 mg
- Frequency
- Once daily
- Route
- SubQ
- Duration
- 6 days (days 15-20)
Morning or early afternoon. Most subjective changes appear after 10-14 days of daily use.
10-20 day cycles (extendable to 28 days), 2-3 cycles per year, with 2-3 months between cycles.
Reconstitution & Storage
1 unit on a U-100 syringe ≈ 66.7 mcg; swirl gently, do not shake. Oral capsule form also exists.
Lyophilized: -20°C long-term or 2-8°C short-term. Reconstituted: 2-8°C. Avoid freeze-thaw cycles.
Benefits
- • Reported reduction of reactive oxygen species in neural cells
- • Restoration of dendritic spines in Alzheimer's animal models
- • Improvements in memory and mental clarity in small TBI cohorts
- • Reported sleep quality improvements (often first noticeable effect)
- • Possible mood and stress-resilience effects
Side effects
- • Mild headache early in cycle
- • Vivid dreams or altered sleep depth
- • Mild GI effects (oral route)
- • Injection-site redness or itching
- • Long-term safety unmapped in Western trials
Contraindications
- • Pregnancy and breastfeeding
- • Active or recent cancer history
- • Seizure disorders
- • Children and adolescents
- • Concurrent CNS prescription medications
- • Known peptide allergies
Gender notes
Men
Same dosing schedule. Some users pair with Epitalon for combined longevity protocol.
Women
Same dosing schedule. Hormonal effects not documented but caution advised during reproductive years.
Research
- Neuroprotective Effects of Tripeptides — Epigenetic Regulators in Mouse Model of Alzheimer's Disease ↗
EDR tripeptide treatment restored dendritic spine density (~71% increase) and reduced amyloid-beta toxicity markers in transgenic Alzheimer's mouse models.
Pharmaceuticals (MDPI) · 2021
- EDR Peptide: Possible Mechanism of Gene Expression and Protein Synthesis Regulation Involved in the Pathogenesis of Alzheimer's Disease ↗
Khavinson group review proposing the mechanism by which Pinealon enters the nucleus and binds promoter regions to modulate antioxidant and neurotrophic gene expression.
International Journal of Molecular Sciences · 2020
- Peptide regulation of cell differentiation, gene expression and protein synthesis ↗
Khavinson review of short peptide bioregulators (including Pinealon) and their proposed mechanisms in aging and CNS function, summarizing decades of Russian preclinical data.
Bulletin of Experimental Biology and Medicine · 2014
Stacks well with
Track Pinealon doses in the app
Built-in reconstitution calculator, dose log, and reminders. Free on Android.
Get on Google PlayEducational use only. Not medical advice. Many peptides shown are not FDA-approved and remain research compounds. Always consult a qualified healthcare provider.